Team:Newcastle/9 July 2010

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==Protocol==
==Protocol==
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* [[TeamNewcastleTransformation of E. coli|Transformation of ''E. coli'']] DH5alpha pSB1AT3 containing ''lacI'' insert.
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* [[TeamNewcastleTransformation of E. coli|Transformation of ''E. coli'']] DH5α pSB1AT3 containing ''lacI'' insert.
==Inference==
==Inference==

Revision as of 13:38, 11 August 2010

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9 July 2010

Contents

LacI BioBrick Construction

Aims

  • To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).

Materials

  • Competent E. coli (DH5alpha strain)
  • Ligation of lacI into pSB1AT3

Protocol

Inference

  • Ecoli DH5alpha is used as a bioreactor to replicate our plasmids to create a large stock ready for extraction from cells.
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