Team:Newcastle/9 July 2010

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=LacI BioBrick Construction=
 
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==Aims==
 
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* To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
 
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==Materials==
 
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* Competent ''E. coli'' (DH5alpha strain)
 
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* Ligation of ''lacI'' into pSB1AT3
 
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==Protocol==
 
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* [[TeamNewcastleTransformation of E. coli|Transformation of ''E. coli'']] DH5α with pSB1AT3 containing ''lacI'' insert.
 
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==Inference==
 
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*''Ecoli'' DH5alpha is used as a bioreactor to replicate our plasmids to create a large stock ready for extraction from cells.
 
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Revision as of 19:09, 25 October 2010

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