Latest revision as of 19:16, 25 October 2010

Research

Today we initiated with our research in urease which would lead to calcium carbonate production inside the crack.

Wray LV, Ferson E and Fisher SH. (1997). "Expression of the Bacillus subtilis ureABC operon is controlled by multiple regulatory factors including CodY, GlnR, TnrA, and Spo0H". Journal of bacteriology. 179(17). 5494-501.
Kim JK, Mulrooney SB and Hausinger RP. (2005). "Biosynthesis of Active Bacillus subtilis Urease in the Absence of Known Urease Accessory Proteins". Journal of Bacteriology. 187(20). 7150-7154.

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-'''9 July 2010'''
+=Research=
-=LacI BioBrick Construction=
+Today we initiated with our research in urease which would lead to calcium carbonate production inside the crack.
-==Aims==
+#Wray LV, Ferson E and Fisher SH. (1997). "Expression of the Bacillus subtilis ureABC operon is controlled by multiple regulatory factors including CodY, GlnR, TnrA, and Spo0H". ''Journal of bacteriology''. 179(17). 5494-501.
-* To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
+#Kim JK, Mulrooney SB and Hausinger RP. (2005). "Biosynthesis of Active Bacillus subtilis Urease in the Absence of Known Urease Accessory Proteins". ''Journal of Bacteriology''. 187(20). 7150-7154.
-==Materials==
-* Competent ''E. coli'' (DH5alpha strain)
-* Ligation of ''lacI'' into pSB1AT3
-==Protocol==
-* [[TeamNewcastleTransformation of E. coli|Transformation of ''E. coli'']] DH5α pSB1AT3 containing ''lacI'' insert.
-==Inference==
-*''Ecoli'' DH5alpha is used as a bioreactor to replicate our plasmids to create a large stock ready for extraction from cells.
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