Team:Newcastle/9 August 2010

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Contents

Transformation

Results/Aims

  • pVeg transformed successfully- From this we will set up an overnight broth culture for plasmid extraction
  • lacI- To show that we have sucessfully transformed from lacI we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture.

Ligation

Aims

Today we will transform some competent E.coli DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on 06.08.10.

Transformation from ligation products

Aims

To transform some competent E.coli DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on 06.08.10.

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