Team:Newcastle/9 August 2010

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(Difference between revisions)
(Results/Aims)
(Transformation of pVeg and lacI)
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==Results/Aims==  
==Results/Aims==  
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* pVeg was successfully transformed - From this we will set up an [[Team:Newcastle/Growing_an_overnight_cultures|overnight broth culture]] for [[Team:Newcastle/Qiagen_Minipreps|plasmid extraction]]
+
* There are colonies on all 3 plates for pVeg.
-
* ''lacI'' - To show that we have sucessfully transformed from ''lacI'' we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures.
+
* For ''lacI'', there is only one colony on one of the normal plates and more on the concentrated one.
 +
 
 +
==Discussion==
 +
 
 +
* For pVeg, we will set up an [[Team:Newcastle/Growing_an_overnight_cultures|overnight broth culture]] from the colonies we got on the plates for [[Team:Newcastle/Qiagen_Minipreps|plasmid extraction]]
 +
* To show that we have sucessfully transformed from ''lacI'', we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures.
=Transformation of lacI=
=Transformation of lacI=

Revision as of 10:54, 9 August 2010

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Contents

Transformation of pVeg and lacI

Results/Aims

  • There are colonies on all 3 plates for pVeg.
  • For lacI, there is only one colony on one of the normal plates and more on the concentrated one.

Discussion

  • For pVeg, we will set up an overnight broth culture from the colonies we got on the plates for plasmid extraction
  • To show that we have sucessfully transformed from lacI, we will select 4 colonies and plate them on a section of an agar plate as well as setting up overnight broth cultures.

Transformation of lacI

Aims

To transform some competent E.coli DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on 06.08.10.

Materials and Protocol

Please refer to Transformation of E.coli.

Result

Please refer to 10.08.10 for result.

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