Team:Newcastle/9 August 2010

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(Difference between revisions)
(Materials and Protocol)
(Materials and Protocol)
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Please refer to [[TeamNewcastleTransformation_of_E._coli|Transformation of E.coli]].
Please refer to [[TeamNewcastleTransformation_of_E._coli|Transformation of E.coli]].
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==Result==
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Please refer to [[Team:Newcastle/10_August_2010|10.08.10]] for result.
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Revision as of 10:20, 9 August 2010

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Contents

Transformation

Results/Aims

  • pVeg transformed successfully- From this we will set up an overnight broth culture for plasmid extraction
  • lacI- To show that we have sucessfully transformed from lacI we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture.

Transformation of lacI

Aims

To transform some competent E.coli DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on 06.08.10.

Materials and Protocol

Please refer to Transformation of E.coli.

Result

Please refer to 10.08.10 for result.

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