Team:Newcastle/9 August 2010

From 2010.igem.org

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(Aims)
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=Transformation=  
=Transformation=  
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==Results/Aims==  
==Results/Aims==  
*pVeg transformed successfully- From this we will set up an overnight broth culture for plasmid extraction
*pVeg transformed successfully- From this we will set up an overnight broth culture for plasmid extraction
*''lacI''- To show that we have sucessfully transformed from ''lacI'' we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture.
*''lacI''- To show that we have sucessfully transformed from ''lacI'' we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture.
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=Ligation=
 
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==Aims==
 
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Today we will transform some competent ''E.coli'' DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on [[Team:Newcastle/6_August_2010|06.08.10]].
 
=Transformation from ligation products=
=Transformation from ligation products=

Revision as of 10:15, 9 August 2010

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Contents

Transformation

Results/Aims

  • pVeg transformed successfully- From this we will set up an overnight broth culture for plasmid extraction
  • lacI- To show that we have sucessfully transformed from lacI we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture.

Transformation from ligation products

Aims

To transform some competent E.coli DH5α with the ligation products: 1:3, 1:5 and the vector from the ligation on 06.08.10.

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