Team:Newcastle/9 August 2010

From 2010.igem.org

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(Results/Aims)
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==Results/Aims==  
==Results/Aims==  
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*pVeg transformed sucessfully- From this we will set up an overnight broth culture for plasmid extraction
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*pVeg transformed successfully- From this we will set up an overnight broth culture for plasmid extraction
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*''lacI''- To show that we have sucessfully transformed form ''lacI'' we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture.  
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*''lacI''- To show that we have sucessfully transformed form ''lacI'' we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture.
=Ligation=
=Ligation=

Revision as of 09:55, 9 August 2010

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Contents

Transformation

Results/Aims

  • pVeg transformed successfully- From this we will set up an overnight broth culture for plasmid extraction
  • lacI- To show that we have sucessfully transformed form lacI we will select 12 colonies and plate them on a section of an agar plate as well as setting up an overnight broth culture.

Ligation

Aims

Today we will transform some competent E.coli DH5alpha with the ligation products: 1:5,1:3 and the vector

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