Latest revision as of 19:08, 25 October 2010

Research

Today we conducted research in the area of swarming which would allow the bacteria to move down the cracks.

Kearns DB, Chu F, Rudner R, and Losick R. (2004). "Genes governing swarming in Bacillus subtilis and evidence for a phase variation mechanism controlling surface motility". Molecular microbiology. 52(2). 357-69.
Julkowska D, Obuchowski M, Holland IB, and Se SJ. (2005). "Comparative Analysis of the Development of Swarming Communities of Bacillus subtilis 168 and a Natural Wild Type : Critical Effects of Surfactin and the Composition of the Medium". Journal of Bacteriology. 187(1). 65-76.

@@ Line 1: / Line 1: @@
 {{Team:Newcastle/mainbanner}}
-=LacI BioBrick Construction=
+=Research=
-==Aims==
+Today we conducted research in the area of swarming which would allow the bacteria to move down the cracks.
-*To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (''rfp'').
-==Materials==
+#Kearns DB, Chu F, Rudner R, and Losick R. (2004). "Genes governing swarming in Bacillus subtilis and evidence for a phase variation mechanism controlling surface motility". ''Molecular microbiology''. 52(2). 357-69.
-* Digested ''lacI''
+#Julkowska D, Obuchowski M, Holland IB, and Se SJ. (2005). "Comparative Analysis of the Development of Swarming Communities of Bacillus subtilis 168 and a Natural Wild Type : Critical Effects of Surfactin and the Composition of the Medium". ''Journal of Bacteriology''. 187(1). 65-76.
-* Excised gel containing pSB1AT3
-==Protocol==
-* ''lacI'' digest is run on an [[Team:Newcastle/Gel_electrophoresis|agarose gel]] and excised
-* Excised pSB1AT3 and ''lacI'' is then gel extracted and purified.
-* pSB1AT3 and ''lacI'' are [[Team:Newcastle/Ligation|ligated]]
-==Inference==
-* ''lacI'' digest is purified by running on the agarose gel, removing unwanted fragments with homologous sticky ends. It is then ligated into vector pSB1AT3 ready to be transformed into ''E. Coli'' DH5α.
 {{Team:Newcastle/footer}}