Team:Newcastle/5 July 2010

From 2010.igem.org

Revision as of 10:59, 2 August 2010 by PhilipHall (Talk | contribs)

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

LacI BioBrick Construction

Aims

  • To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).

Materials

Protocol

  • Set up a liquid culture of colonies transformed with pMutin4 and pSB1AT3

Inference

  • Transformed Ecoli DH5α grown up overnight to create a large stock of plasmid ready for extraction
Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon