Team:Newcastle/5 July 2010

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Contents

LacI BioBrick Construction

Aims

  • To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).

Materials

  • Refrigerated transformation plates

Protocol

  • Set up a liquid culture of colonies transformed with pMutin4 and pSB1AT3

Inference

  • Transformed Ecoli DH5alpha grown up overnight to create a large stock of plasmid ready for extraction
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