Team:Newcastle/5 July 2010

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(LacI BioBrick Construction)
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==LacI BioBrick Construction==
 
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===Aims===
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'''5 July 2010'''
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=LacI BioBrick Construction=
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==Aims==
*To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
*To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
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===Materials===
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==Materials==
* Refrigerated [[Team:Newcastle/1_July_2010#LacI_BioBrick_Construction|transformation plates]].
* Refrigerated [[Team:Newcastle/1_July_2010#LacI_BioBrick_Construction|transformation plates]].
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===Protocol===
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==Protocol==
* Set up a [[Team:Newcastle/Growing_an_overnight_cultures|overnight culture]] of colonies transformed with pMutin4 and pSB1AT3.
* Set up a [[Team:Newcastle/Growing_an_overnight_cultures|overnight culture]] of colonies transformed with pMutin4 and pSB1AT3.
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===Inference===
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==Inference==
*Transformed ''E. coli'' DH5α grown up overnight to create a large stock of plasmid ready for extraction.
*Transformed ''E. coli'' DH5α grown up overnight to create a large stock of plasmid ready for extraction.
{{Team:Newcastle/footer}}
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Revision as of 10:49, 11 August 2010

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5 July 2010

Contents

LacI BioBrick Construction

Aims

  • To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).

Materials

Protocol

Inference

  • Transformed E. coli DH5α grown up overnight to create a large stock of plasmid ready for extraction.
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