Revision as of 17:05, 25 October 2010

Research

Today we started off our research on filamentous cells which will act as reinforcing fibres in the crack.

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-=LacI BioBrick Construction=
+=Research=
+Today we started off our research on filamentous cells which will act as reinforcing fibres in the crack.
-==Aims==
+#
-*To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (''rfp'').
-==Materials==
-*Miniprep of pSB1AT3.
-==Protocol==
-* Set up a [[Team:Newcastle/Growing_an_overnight_cultures|overnight culture]] of colonies transformed with pSB1AT3 to prepare stocks.
-==Inference==
-*Transformed ''E. coli'' DH5α grown up overnight to create a large stock of plasmid ready for extraction.
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