Team:Newcastle/5 July 2010

From 2010.igem.org

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(Aims)
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==Aims==
==Aims==
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*To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).
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*To use PCR to extract ''lacI'' (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (''rfp'').
==Materials==
==Materials==

Revision as of 15:02, 25 October 2010

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Contents

LacI BioBrick Construction

Aims

  • To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (rfp).

Materials

Protocol

Inference

  • Transformed E. coli DH5α grown up overnight to create a large stock of plasmid ready for extraction.
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