Team:Newcastle/5 July 2010

From 2010.igem.org

(Difference between revisions)
Line 1: Line 1:
{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/mainbanner}}
-
 
-
'''5 July 2010'''
 
=LacI BioBrick Construction=
=LacI BioBrick Construction=

Revision as of 14:19, 25 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

LacI BioBrick Construction

Aims

  • To use PCR to extract lacI (promoter, ribosome-binding site (RBS) & coding sequence (CDS)) from plasmid pMutin4 and ligate into vector pSB1AT3 in front of red fluorescent protein (RFP).

Materials

Protocol

Inference

  • Transformed E. coli DH5α grown up overnight to create a large stock of plasmid ready for extraction.
Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon