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Team:Newcastle/4 August 2010
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Contents |
Cloning the rocF BioBrick
Aim
The aim of today's experiment is to amplify 6 different fragments for the construction of rocF BioBrick with the help of 6 different Phusion PCR.
Materials and Protocol
Please refer to PCR for Phusion PCR protocol. The details for the 6 PCR reactions are mentioned below:
PCR
| pSB1C3
(No. 1) | pSB1C3
(No. 2) | pSB1C3
(No. 3) | pSB1C3
(No. 4) | lacI
(No. 1) | lacI
(No. 2) | Double terminator !Double terminator
(No. 1) | Double terminator
(No. 2) |
|---|---|---|---|---|---|---|---|
| 44.0 µl/ml | 19.9 µl/ml | 25.0 µl/ml | 30.8 µl/ml | 10.0 µl/ml | 44.2 µl/ml | 9.2 µl/ml | 39.7 µl/ml |
Gibson assembly
   
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