Revision as of 09:20, 5 August 2010

Cloning the rocF BioBrick

The aim of today's experiment is to amplify 6 different fragments for the construction of rocF BioBrick with the help of 6 different Phusion PCR.

Please refer to PCR for Phusion PCR protocol. The details for the 6 PCR reactions are mentioned below:

Tube	Part to be amplified	Plasmid consisting the part	Forward primer	Reverse Primer	Melting Temperature (Tm in °C)	Size of the fragment (in bp)	*Extension time (in seconds)**
1	Plasmid Vector	pSB1C3	P1V1 forward	P2V1 reverse	58	2072 approx.	60
2	Pspacoid Promoter	pMutin4	P1P1 forward	P2P1 reverse	49	106 approx.	15

@@ Line 29: / Line 29: @@
 |2072 approx.
 |60
+|-
+|2
+|Pspacoid Promoter
+|pMutin4
+|P1P1 forward
+|P2P1 reverse
+|49
+|106 approx.
+|15
 |}