Team:Newcastle/3 September 2010

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(Results)
(Results)
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[[Image:03.09.10.png#file|400px]]
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'''Figure 1''':  
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'''Figure 1''': Restriction digest result of pGFPrrnB with
* '''Lane 1''': 1kb DNA ladder
* '''Lane 1''': 1kb DNA ladder

Revision as of 00:51, 26 October 2010

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Contents

yneA

Aims

The aim of the experiment is to test for the correct integration of yneA in pGFPrrnb plasmid.

Materials and protocols

  1. Please refer to: Plasmid extraction.
  2. Please refer to:Restriction digest. We used Nhe1 and Spe1 to remove the yneA from pGFP-rrnB.
  3. Please refer to: Gel electrophoresis for running all the digested plasmid fragments.

Results

03.09.10.png

Figure 1: Restriction digest result of pGFPrrnB with

  • Lane 1: 1kb DNA ladder
  • Lane 2: Tube 1
  • Lane 3: Tube 2
  • Lane 4: Tube 3
  • Lane 5: Tube 4
  • Lane 6: Tube 5
  • Lane 7: Tube 6
  • Lane 8: Tube 7
  • Lane 9: Tube 8
  • Lane 10: Tube 9
  • Lane 11: Tube 10
  • Lane 12: Tube 11
  • Lane 13: Tube 12
  • Lane 14: 1kb DNA ladder

Conclusion

The results show that the digest works, there is a band at approximately 541bp corresponding to yneA and a band at approximately 8.4kbp corresponding to pGFPrrnb in lanes 2,3,4,6,7,8,9, 11 and 12.


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