From 2010.igem.org

Arginine consists of a positive charge on it and thus is acidic in nature. As the cells take up the arginine from the media, the pH of the media increases. The tube labeled Negative control contains no cells and the pH of the media has remained constant for 5 hours which shows that the level of arginine in the media has remained constant, while the tube labeled Test which contains Bacillus subtilis 168 cells shows an increase in pH which shows that the level of arginine has decreased in the media.

Conclusion

It can be concluded that the Bacillus subtilis 168 cells are able to take up arginine from the media into the cells.

@@ Line 1: / Line 1: @@
-===Aim of this experiment===
+{{Team:Newcastle/mainbanner}}
+=Arginine Test=
+==Aims==
 The aim of this experiment is to prove that ''Bacillus subtilis'' 168 can take up arginine from the media into the cell.
-===Materials Required===
+==Materials and Protocol==
-* pH strips
+Please refer to: [[Team:Newcastle/Arginine test| Arginine test]] for materials required and protocol.
-* LB media consisting arginine and ampicillin
-* Auto pipette
+==Result==
-* Gilson pipette
+{|border=1
-* Autoclaved tips for gilson pipette
+|-
-* Burnsen Burner
+!Hours
-* Universal Tube
+!Negative control
+!Test
+|-
+|0
+|pH 7
+|pH 7
+|-
+|1
+|pH 7
+|pH 7
+|-
+|2
+|pH 7
+|pH 7.5
+|-
+|3
+|pH 7
+|pH 7.5
+|-
+|4
+|pH 7
+|pH 8
+|-
+|5
+|pH 7
+|pH 8
+|}
+==Discussion==
+Arginine consists of a positive charge on it and thus is acidic in nature. As the cells take up the arginine from the media, the pH of the media increases. The tube labeled Negative control contains no cells and the pH of the media has remained constant for 5 hours which shows that the level of arginine in the media has remained constant, while the tube labeled Test which contains ''Bacillus subtilis'' 168 cells shows an increase in pH which shows that the level of arginine has decreased in the media.
+==Conclusion==
+It can be concluded that the ''Bacillus subtilis'' 168 cells are able to take up arginine from the media into the cells.
-===Procedure===
-* Label the universal tube as "Overnight Culture".
-* Unscrew the caps of the universal tube and after unscrewing, flame the mouth of the tube.
-* With the help of an auto pipette, pipette out 5ml of LB broth from the stock solution and transfer it into universal tubes. Do this process three times so as to transfer 5ml of LB media in all the three tubes.
-* Flame the flaming loop with the help of a burner till the loop gets red in colour.
-* Allow it to cool by holding the loop for approximately 10 seconds.
-* Use the loop to pick up a colony from an agar plate consisting of ''Bacillus subtilis'' 168 colonies and transfer the loop into the universal tube consisting the LB media.
-* Flame the mouth of the universal tubes and screw the cap (do not over tight the cap so as to allow oxygen to enter the tube)and shake gently so as to suspend the cells equally.
-* Flame the flaming loop with the help of a burner till the loop gets red in colour so as to kill the cells present on it.
-* Put the universal tube on a shaker at 37° C and leave it overnight for the cells to grow.
-===Inference===
+{{Team:Newcastle/footer}}
-Cells will grow overnight at an exponential phase in the universal tube consisting media.

Team:Newcastle/29 June 2010

From 2010.igem.org

Latest revision as of 22:15, 21 October 2010

Contents

Arginine Test

Aims

Materials and Protocol

Result

Discussion

Conclusion