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From 2010.igem.org

Aim of this experiment

The aim of this experiment was to prove that Bacillus subtilis 168 can take up arginine from the media into the cell.

Materials Required

• Plate consisting of Bacillus subtilis 168 colonies.
• Flaming (streaking) Loop
• LB media
• Auto pipette
• Bursen Burner
• Universal Tubes

Procedure

• Unscrew the caps of three universal tubes and after unscrewing, flame the mouth of the tube.
• With the help of an auto pipette, pipette out 5ml of LB broth from the stock solution and transfer it into universal tubes. Do this process three times so as to transfer 5ml of LB media in all the three tubes.
• Flame the flaming loop with the help of a burner till the loop gets red in colour.
• Allow it to cool by holding the loop for approximately 10 seconds.
• Use the loop to pick up a colony from an agar plate consisting of Bacillus subtilis 168 colonies and transfer the loop into the universal tube consisting the LB media.
• Flame the mouth of the universal tubes and screw the cap and shake gently so as to suspend the cells equally.
• Flame the flaming loop with the help of a burner till the loop gets red in colour so as to kill the cells present on it.