"
Team:Newcastle/28 June 2010
From 2010.igem.org
Revision as of 13:37, 21 July 2010 by Shethharsh08 (Talk | contribs)
Aim of this experiment
The aim of this experiment was to prove that Bacillus subtilis 168 can take up arginine from the media into the cell.
Materials Required
- Plate consisting of Bacillus subtilis 168 colonies.
- Flaming (streaking) Loop
- LB media
- Auto pippette
- Bursen Burner
- Universal Tubes
Procedure
- Flame the flaming loop with the help of a burner till the loop gets red in colour.
- Allow it to cool by holding the loop for approximately 10 seconds.
- Use the loop to pick up a colony from an agar plate consisting of Bacillus subtilis 168 colonies.
