"
Team:Newcastle/28 June 2010
From 2010.igem.org
(Difference between revisions)
Shethharsh08 (Talk | contribs) |
Shethharsh08 (Talk | contribs) |
||
| Line 2: | Line 2: | ||
The aim of this experiment was to prove that ''Bacillus subtilis'' 168 can take up arginine from the media into the cell. | The aim of this experiment was to prove that ''Bacillus subtilis'' 168 can take up arginine from the media into the cell. | ||
| - | ===Materials | + | ===Materials Required=== |
* Plate consisting of ''Bacillus subtilis'' 168 colonies. | * Plate consisting of ''Bacillus subtilis'' 168 colonies. | ||
* Flaming (streaking) Loop | * Flaming (streaking) Loop | ||
| Line 9: | Line 9: | ||
* Bursen Burner | * Bursen Burner | ||
* Universal Tubes | * Universal Tubes | ||
| + | |||
| + | ===Procedure=== | ||
| + | * Flame the flaming loop with the help of a burner till the loop gets red in colour. | ||
| + | * Allow it to cool by holding the loop for approximately 10 seconds. | ||
| + | * Use the loop to pick up a colony from an agar plate consisting of ''Bacillus subtilis'' 168 colonies. | ||
| + | * | ||
Revision as of 13:37, 21 July 2010
Aim of this experiment
The aim of this experiment was to prove that Bacillus subtilis 168 can take up arginine from the media into the cell.
Materials Required
- Plate consisting of Bacillus subtilis 168 colonies.
- Flaming (streaking) Loop
- LB media
- Auto pippette
- Bursen Burner
- Universal Tubes
Procedure
- Flame the flaming loop with the help of a burner till the loop gets red in colour.
- Allow it to cool by holding the loop for approximately 10 seconds.
- Use the loop to pick up a colony from an agar plate consisting of Bacillus subtilis 168 colonies.
