Team:Newcastle/28 June 2010

From 2010.igem.org

(Difference between revisions)
(Inference)
 
(24 intermediate revisions not shown)
Line 1: Line 1:
-
===Aim of this experiment===
+
{{Team:Newcastle/mainbanner}}
-
The aim of this experiment was to prove that ''Bacillus subtilis'' 168 can take up arginine from the media into the cell.
+
-
===Materials Required===
+
'''28 June 2010'''
-
* Plate consisting of ''Bacillus subtilis'' 168 colonies.
+
-
* Flaming (streaking) Loop
+
-
* LB media
+
-
* Auto pippette
+
-
* Bursen Burner
+
-
* Universal Tubes
+
-
===Procedure===
+
==Aims==
-
* Flame the flaming loop with the help of a burner till the loop gets red in colour.
+
To prove that ''Bacillus subtilis'' 168 can take up arginine from the media into the cell.
-
* Allow it to cool by holding the loop for approximately 10 seconds.
+
 
-
* Use the loop to pick up a colony from an agar plate consisting of ''Bacillus subtilis'' 168 colonies.
+
==Materials==
-
*
+
* ''Bacillus subtilis'' 168  
 +
* LB media
 +
* arginine
 +
* Universal Tube
 +
 
 +
==Protocol==
 +
[[Team:Newcastle/Growing_an_overnight_cultures|Set up an overnight culture]]
 +
 
 +
==Inference==
 +
Cells will grow overnight in the universal tube consisting media.
 +
 
 +
 
 +
{{Team:Newcastle/footer}}

Latest revision as of 11:12, 27 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

28 June 2010

Contents

Aims

To prove that Bacillus subtilis 168 can take up arginine from the media into the cell.

Materials

  • Bacillus subtilis 168
  • LB media
  • arginine
  • Universal Tube

Protocol

Set up an overnight culture

Inference

Cells will grow overnight in the universal tube consisting media.


Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon