Team:Newcastle/28 July 2010
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=Plasmid Miniprep Experiment= | =Plasmid Miniprep Experiment= | ||
+ | |||
+ | ==Aims== | ||
+ | The aim of this experiment is to prove that ''Bacillus subtilis'' 168 and 3610 DNA extraction worked by amplifying ''anaR'' gene using PCR. | ||
+ | |||
+ | ==Materials and Protocol== | ||
+ | |||
+ | Please refer to: [[Team:Newcastle/PCR| PCR]] for GoTaq PCR. | ||
+ | |||
+ | ==Result== | ||
+ | * '''Lane 1''': 1kb DNA ladder | ||
+ | * '''Lane 2''': ''B. subtilis'' 168 chromosomal DNA containing ''anaR'' gene | ||
+ | * '''Lane 3''': ''B. subtilis'' 168 chromosomal DNA containing ''anaR'' gene | ||
+ | * '''Lane 4''': ''B. subtilis'' 3610 chromosomal DNA containing ''anaR'' gene | ||
+ | * '''Lane 5''': ''B. subtilis'' 3610 chromosomal DNA containing ''anaR'' gene | ||
+ | |||
+ | ==Discussion== | ||
+ | We found bands in the lane 2, 3, 4 and 5 of around 200 bp size which is an approximate size of the ''anaR'' gene which is found on the chromosome of both ''B. subtilis'' 168 and 3610. | ||
+ | |||
+ | ==Conclusion== | ||
+ | This experiment proves that the DNA extraction from both ''B. subtilis'' 168 and 3610 done on 27th July, 2010 was successful. | ||
+ | |||
{{Team:Newcastle/footer}} | {{Team:Newcastle/footer}} |
Revision as of 21:42, 28 July 2010
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Contents |
PCR Experiment
Aims
The aim of this experiment is to prove that Bacillus subtilis 168 and 3610 DNA extraction worked by amplifying anaR gene using PCR.
Materials and Protocol
Please refer to: PCR for GoTaq PCR.
Result
- Lane 1: 1kb DNA ladder
- Lane 2: B. subtilis 168 chromosomal DNA containing anaR gene
- Lane 3: B. subtilis 168 chromosomal DNA containing anaR gene
- Lane 4: B. subtilis 3610 chromosomal DNA containing anaR gene
- Lane 5: B. subtilis 3610 chromosomal DNA containing anaR gene
Discussion
We found bands in the lane 2, 3, 4 and 5 of around 200 bp size which is an approximate size of the anaR gene which is found on the chromosome of both B. subtilis 168 and 3610.
Conclusion
This experiment proves that the DNA extraction from both B. subtilis 168 and 3610 done on 27th July, 2010 was successful.
Plasmid Miniprep Experiment
Aims
The aim of this experiment is to prove that Bacillus subtilis 168 and 3610 DNA extraction worked by amplifying anaR gene using PCR.
Materials and Protocol
Please refer to: PCR for GoTaq PCR.
Result
- Lane 1: 1kb DNA ladder
- Lane 2: B. subtilis 168 chromosomal DNA containing anaR gene
- Lane 3: B. subtilis 168 chromosomal DNA containing anaR gene
- Lane 4: B. subtilis 3610 chromosomal DNA containing anaR gene
- Lane 5: B. subtilis 3610 chromosomal DNA containing anaR gene
Discussion
We found bands in the lane 2, 3, 4 and 5 of around 200 bp size which is an approximate size of the anaR gene which is found on the chromosome of both B. subtilis 168 and 3610.
Conclusion
This experiment proves that the DNA extraction from both B. subtilis 168 and 3610 done on 27th July, 2010 was successful.