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Team:Newcastle/28 July 2010
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==Result== | ==Result== | ||
* '''Lane 1''': 1kb DNA ladder | * '''Lane 1''': 1kb DNA ladder | ||
| - | * '''Lane 2''': ''B. subtilis'' 168 chromosomal DNA'' | + | * '''Lane 2''': ''B. subtilis'' 168 chromosomal DNA containing ''anaR'' gene |
| - | * '''Lane 3''': ''B. subtilis'' 168 chromosomal DNA'' | + | * '''Lane 3''': ''B. subtilis'' 168 chromosomal DNA containing ''anaR'' gene |
| - | * '''Lane 4''': ''B. subtilis'' 3610 chromosomal DNA'' | + | * '''Lane 4''': ''B. subtilis'' 3610 chromosomal DNA containing ''anaR'' gene |
| - | * '''Lane 5''': ''B. subtilis'' 3610 chromosomal DNA'' | + | * '''Lane 5''': ''B. subtilis'' 3610 chromosomal DNA containing ''anaR'' gene |
==Discussion== | ==Discussion== | ||
Revision as of 14:57, 28 July 2010
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Contents |
Aims
The aim of this experiment is to prove that Bacillus subtilis 168 and 3610 DNA extraction worked by amplifying anaR gene using PCR.
Materials and Protocol
Please refer to: PCR for GoTaq PCR.
Result
- Lane 1: 1kb DNA ladder
- Lane 2: B. subtilis 168 chromosomal DNA containing anaR gene
- Lane 3: B. subtilis 168 chromosomal DNA containing anaR gene
- Lane 4: B. subtilis 3610 chromosomal DNA containing anaR gene
- Lane 5: B. subtilis 3610 chromosomal DNA containing anaR gene
Discussion
We found bands in the lane 2,3,4 and 5 of around 200 bp which is an approximate size of the anaR gene which is found on the chromosome of both B. subtilis 168 and 3610.
Conclusion
It can be concluded that the Bacillus subtilis 168 cells are able to take up arginine from the media into the cells.
   
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