Team:Newcastle/28 July 2010

We found bands in the lane 2,3,4 and 5 of around 200 bp which is an approximate size of the anaR gene which is found on the chromosome of both B. subtilis 168 and 3610.

Conclusion

It can be concluded that the Bacillus subtilis 168 cells are able to take up arginine from the media into the cells.

@@ Line 16: / Line 16: @@
 ==Discussion==
-Arginine consists of a positive charge on it and thus is acidic in nature. As the cells take up the arginine from the media, the pH of the media increases. The tube labeled Negative control contains no cells and the pH of the media has remained constant for 5 hours which shows that the level of arginine in the media has remained constant, while the tube labeled Test which contains ''Bacillus subtilis'' 168 cells shows an increase in pH which shows that the level of arginine has decreased in the media.
+We found bands in the lane 2,3,4 and 5 of around 200 bp which is an approximate size of the ''anaR'' gene which is found on the chromosome of both ''B. subtilis'' 168 and 3610.
 ==Conclusion==

Team:Newcastle/28 July 2010

From 2010.igem.org

Revision as of 14:57, 28 July 2010

Contents

Aims

Materials and Protocol

Result

Discussion

Conclusion