Team:Newcastle/27 July 2010

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Contents 1 Genomic DNA extraction experiment 1.1 Aim 1.2 Procedure 1.3 Discussion 1.4 Conclusion 2 Gel Electrophoresis 2.1 Protocol 2.1.1 Materials 2.2 Discussion

Genomic DNA extraction experiment

Aim

The aim of today's experiment is to extract genomic DNA from Bacillus subtilis strain 3610 and 168,genes from which will be needed for the swarming biobrick and rocF biobrick.

Procedure

• Please refer to DNA extraction of Bacillus subtilis

Discussion

At the end of the DNA precipitation step, we did observe a small white pellet in all the eppendorf tubes.

Conclusion

Th experiment was successful and we would check the content and the purity of the extracted DNA by using PCR on 28th July, 2010.

Gel Electrophoresis

Protocol

• Please refer to Gel Electrophoresis

Materials

1. 50ml of 1% Agarose TAE
2. 5µl SafeView
3. TAE Buffer

Discussion