Team:Newcastle/27 July 2010

From 2010.igem.org

(Difference between revisions)
(Genomic DNA extraction experiment)
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==Genomic DNA extraction experiment==
==Genomic DNA extraction experiment==
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[[Image:Newcastle alan chromosome.jpg|thumb|300px|right]]
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[[Image:Newcastle alan chromosome.jpg|thumb|200px|right]]
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[[Image:Newcastle ice chromosome.jpg|thumb|300px|right]]
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[[Image:Newcastle ice chromosome.jpg|thumb|200px|right]]
===Aims===
===Aims===
The aim of today's experiment is to extract genomic DNA from both ''B. subtilis'' strains 168 and 3610. The genes necessary for the swarming biobrick and ''rocF'' biobrick will then hopefully be obtained from the genomic DNA using...
The aim of today's experiment is to extract genomic DNA from both ''B. subtilis'' strains 168 and 3610. The genes necessary for the swarming biobrick and ''rocF'' biobrick will then hopefully be obtained from the genomic DNA using...
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===Conclusion===
===Conclusion===
The experiment was a success! The content and purity of the extracted DNA will be checked by using PCR on 28th July, 2010.
The experiment was a success! The content and purity of the extracted DNA will be checked by using PCR on 28th July, 2010.
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==Gel Electrophoresis==
==Gel Electrophoresis==
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[[Image:P7270470.JPG|thumb|300px|right]]
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[[Image:P7270470.JPG|thumb|200px|right]]
===Protocol===
===Protocol===
* Please refer to the [[Team:Newcastle/Gel electrophoresis|gel electrophoresis]] page for full protocol.
* Please refer to the [[Team:Newcastle/Gel electrophoresis|gel electrophoresis]] page for full protocol.

Revision as of 10:36, 3 August 2010

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Contents

Genomic DNA extraction experiment

Newcastle alan chromosome.jpg
Newcastle ice chromosome.jpg

Aims

The aim of today's experiment is to extract genomic DNA from both B. subtilis strains 168 and 3610. The genes necessary for the swarming biobrick and rocF biobrick will then hopefully be obtained from the genomic DNA using...

Protocol

Discussion

At the end of the DNA precipitation step, we did observe a small white pellet in all the eppendorf tubes.

Conclusion

The experiment was a success! The content and purity of the extracted DNA will be checked by using PCR on 28th July, 2010.


Gel Electrophoresis

P7270470.JPG

Protocol

Discussion

  • Unfortunately no bands were observed on the gel.

Conclusion

As no bands were observed on the gel, the PCR did not work. This could be due to a number of reasons: It could be due to the DNA not being extracted or that we might have used the wrong primers. It could also be caused by the small size of the fragment that we amplified which ran off the gel because we ran the gel for too long.








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