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Team:Newcastle/27 August 2010
From 2010.igem.org
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First transformation of 'Bacillius subtilis 168' with Prrnb-GFP containing YneA
Starch plating
Aims
Yesterday we replica plated Bacillus subtilis 168, that were transformed on Wednesday, onto starch plates. Today we aim to see whether or not the transformed Bacillus subtilis can breakdown starch. If they cannot break down starch there will be no halo when we test with iodine.
Materials
- Starch plates
- Pipette tips
- Iodine
Results
Unfortunately our colonies had halos. Although the insert has integrated, the colonies have antibiotic restistance, the insert has not integrated at the amyE locus.
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