Team:Newcastle/27 August 2010

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(Difference between revisions)
(PCR and digestion controls)
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==Aims==
==Aims==
Since we have recently had problems with both PCR and restriction digests, we set up positive controls to ensure that there is nothing wrong with any of our enzymes or ...
Since we have recently had problems with both PCR and restriction digests, we set up positive controls to ensure that there is nothing wrong with any of our enzymes or ...
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===Materials and Protocol===
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Please refer to the [[Team:Newcastle/Restriction_digests|restriction digest]] and [[Team:Newcastle/Gel_electrophoresis|gel electrophoresis]] protocols.
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==...==
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Revision as of 10:33, 27 August 2010

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Contents

PCR and digestion controls

Aims

Since we have recently had problems with both PCR and restriction digests, we set up positive controls to ensure that there is nothing wrong with any of our enzymes or ...

Materials and Protocol

Please refer to the restriction digest and gel electrophoresis protocols.


...

Results

Newcastle controlgel 270810.jpg ...

Discussion

Worked correctly.. nothing is wrong with our enzymes.. in the case of our rocF plasmid PCRs, it must be the primers which are the problem.. new ones are supposed to be arriving today.. in the other cases, repeat..

yneA

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