Latest revision as of 01:17, 28 October 2010

Microscope investigations of filamentous cells

We spent today looking at our filamentous cells under the microscope.

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-=PCR and digestion controls=
+=Microscope investigations of filamentous cells=
+We spent today looking at our filamentous cells under the microscope.
-==Aims==
-Since we have recently had problems with both PCR and restriction digests, we set up positive controls to ensure that there is nothing wrong with any of our enzymes or ...
-===Materials and Protocols===
-Please refer to the [[Team:Newcastle/Restriction_digests|restriction digest]], [[Team:Newcastle/PCR#Phusion_PCR|Phusion PCR]] and [[Team:Newcastle/Gel_electrophoresis|gel electrophoresis]] protocols.
-==...==
-==Results==
-[[Image:Newcastle_controlgel_270810.jpg|500px|centre]]
-'''Figure 1''': Gel electrophoresis of the amplified PCR products and restriction digest
-*'''Lane 1''': 1 Kb ladder
-*'''Lane 2''': digested (linearised) pGFP-rrnB
-*'''Lane 3''': PCR product of pSB1C3
-*'''Lane 4''': PCR product of first fragment of ''rocF'' coding sequence
-*'''Lane 5''': 1 Kb ladder
-==Discussion==
-Worked correctly.. nothing is wrong with our enzymes.. in the case of our ''rocF'' plasmid PCRs, it must be the primers which are the problem.. new ones are supposed to be arriving today.. in the other cases, repeat..
-=''yneA''=
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