Team:Newcastle/27 August 2010

From 2010.igem.org

(Difference between revisions)
(yneA)
 
(2 intermediate revisions not shown)
Line 1: Line 1:
{{Team:Newcastle/mainbanner}}
{{Team:Newcastle/mainbanner}}
-
 
+
=Microscope investigations of filamentous cells=
-
 
+
We spent today looking at our filamentous cells under the microscope.
-
=First transformation of 'Bacillius subtilis 168' with Prrnb-GFP containing YneA=
+
-
 
+
-
==Starch plating==
+
-
 
+
-
===Aims===
+
-
 
+
-
Yesterday we replica plated ''Bacillus subtilis'' 168, that were transformed on Wednesday, onto starch plates.
+
-
Today we aim to see whether or not the transformed ''Bacillus subtilis'' can breakdown starch. If they cannot break down starch there will be no halo when we test with iodine.
+
-
 
+
-
===Materials===
+
-
 
+
-
*Starch plates
+
-
*Pipette tips
+
-
*Iodine
+
-
 
+
-
===Results===
+
-
 
+
-
Unfortunately our colonies had halos. Although the insert has integrated, the colonies have antibiotic restistance, the insert has not integrated at the ''amyE'' locus.  
+
-
 
+
-
<center>
+
-
{|
+
-
|-
+
-
|[[Image:Starchplate.jpg|300px|centre]]
+
-
+
-
|[[Image:Starchplate2.jpg|300px|centre]]
+
-
|}
+
-
</center>
+
-
 
+
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 01:17, 28 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Microscope investigations of filamentous cells

We spent today looking at our filamentous cells under the microscope.

Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon