Team:Newcastle/27 August 2010

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-=First transformation of 'Bacillius subtilis 168' with Prrnb-GFP containing YneA=
-==Starch plating==
-===Aims===
-Yesterday we replica plated ''Bacillus subtilis'' 168, that were transformed on Wednesday, onto starch plates.
-Today we aim to see whether or not the transformed ''Bacillus subtilis'' can breakdown starch. If they cannot break down starch there will be no halo when we test with iodine.
-===Materials===
-*Starch plates
-*Pipette tips
-*Iodine
-===Results===
-Unfortunately our colonies had halos. Although the insert has integrated, the colonies have antibiotic restistance, the insert has not integrated at the ''amyE'' locus.
-<center>
-{|
-|-
-|[[Image:Starchplate.jpg|300px|centre]]
-|
-|[[Image:Starchplate2.jpg|300px|centre]]
-|}
-</center>
 {{Team:Newcastle/footer}}

Revision as of 03:33, 26 October 2010