Team:Newcastle/25 June 2010

From 2010.igem.org

(Difference between revisions)
(Result)
 
(10 intermediate revisions not shown)
Line 1: Line 1:
-
==Polymerase Chain Reaction protocol==
+
{{Team:Newcastle/mainbanner}}
-
Polymerase chain reaction (PCR) is a technique used to amplify a single or few a copies of a piece of DNA generating thousands to millions of copies of a particular DNA sequence.
+
=Polymerase Chain Reaction=
-
# To avoid contamination, wear gloves
+
-
# To achieve optimum results, always do everything on ice
+
-
A basic PCR set up requires several components and reagents.These components include:
+
==Aim==
-
#DNA template that contains the DNA region (target) to be amplified.
+
-
#Two primers that are complementary to the 3' (three prime) ends of each of the sense and anti-sense strand of the DNA target.
+
-
#Taq polymerase or another DNA polymerase with a temperature optimum at around 70 °C.
+
-
#Deoxynucleoside triphosphates, the building blocks from which the DNA polymerases synthesizes a new DNA strand.
+
-
#Buffer solution, providing a suitable chemical environment for optimum activity and stability of the DNA polymerase.
+
-
[[Image:fri-gel.png|400px]] [[Image:Newcastle gel 20100625.jpg|400px]]
+
To amplify a desired DNA fragment by using a set of appropriate primers flanking the fragment.
 +
 
 +
==Materials and Protocol==
 +
 
 +
Please refer to: [[Team:Newcastle/PCR| PCR]] for materials required and protocol.
 +
 
 +
==Result==
 +
 
 +
[[Image:8062010.jpg|400px|center]]
 +
 
 +
'''Image 1''': Image of the gel showing PCR products.
 +
 
 +
* '''Lane 1''': 1kb DNA ladder
 +
* '''Lane 2''': Sample 1
 +
* '''Lane 3''': Sample 2
 +
* '''Lane 4''': Sample 3
 +
* '''Lane 5''': Sample 4
 +
* '''Lane 6''': Sample 5
 +
* '''Lane 7''': Sample 6
 +
* '''Lane 8''': Sample 7
 +
* '''Lane 9''': Sample 8
 +
* '''Lane 10''': Sample 9
 +
* '''Lane 11''': Sample 10
 +
* '''Lane 12''': Sample 11
 +
* '''Lane 13''': Sample 12
 +
* '''Lane 14''': Sample 13
 +
* '''Lane 15''': Sample 14
 +
 
 +
 
 +
 
 +
{{Team:Newcastle/footer}}

Latest revision as of 13:54, 27 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

Polymerase Chain Reaction

Aim

To amplify a desired DNA fragment by using a set of appropriate primers flanking the fragment.

Materials and Protocol

Please refer to: PCR for materials required and protocol.

Result

8062010.jpg

Image 1: Image of the gel showing PCR products.

  • Lane 1: 1kb DNA ladder
  • Lane 2: Sample 1
  • Lane 3: Sample 2
  • Lane 4: Sample 3
  • Lane 5: Sample 4
  • Lane 6: Sample 5
  • Lane 7: Sample 6
  • Lane 8: Sample 7
  • Lane 9: Sample 8
  • Lane 10: Sample 9
  • Lane 11: Sample 10
  • Lane 12: Sample 11
  • Lane 13: Sample 12
  • Lane 14: Sample 13
  • Lane 15: Sample 14


Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon