Team:Newcastle/25 August 2010

From 2010.igem.org

(Difference between revisions)
(Results)
(Results)
Line 20: Line 20:
*'''Lane 1''': Vector only
*'''Lane 1''': Vector only
-
*'''Lane 2''': pGFPrrnB + ''yneA'' 1
+
*'''Lane 2''': Tube 1
-
*'''Lane 3''': pGFPrrnB + ''yneA'' 2
+
*'''Lane 3''': Tube 2
-
*'''Lane 4''': pGFPrrnB + ''yneA'' 3
+
*'''Lane 4''': Tube 3
-
*'''Lane 5''': pGFPrrnB + ''yneA'' 4
+
*'''Lane 5''': Tube 4
-
*'''Lane 6''': pGFPrrnB + ''yneA'' 5
+
*'''Lane 6''': Tube 5
-
*'''Lane 7''': pGFPrrnB + ''yneA'' 6
+
*'''Lane 7''': Tube 6
-
*'''Lane 8''': pGFPrrnB + ''yneA'' 7
+
*'''Lane 8''': Tube 7
-
*'''Lane 9''': pGFPrrnB + ''yneA'' 8
+
*'''Lane 9''': Tube 8
-
*'''Lane 10''': pGFPrrnB + ''yneA'' 9
+
*'''Lane 10''': Tube 9
-
*'''Lane 11''': pGFPrrnB + ''yneA'' 10
+
*'''Lane 11''': Tube 10
-
*'''Lane 12''': pGFPrrnB + ''yneA'' 11
+
*'''Lane 12''': Tube 11
-
*'''Lane 13''': pGFPrrnB + ''yneA'' 12
+
*'''Lane 13''': Tube 12
===Discussion===
===Discussion===

Revision as of 09:31, 26 August 2010

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Contents

yneA

Gel Electrophoresis

Aim

To check if the digestion from yesterday worked.

Materials and Protocol

Please refer to gel electrophoresis for protocol.

Results

The result from gel electrophoresis:

Newcastle 25-08-2010 – Gel 1 (Ethidium Bromide).png

Figure 1 shows the double digest of 12 tubes of pGFPrrnB and yneA.

  • Lane 1: Vector only
  • Lane 2: Tube 1
  • Lane 3: Tube 2
  • Lane 4: Tube 3
  • Lane 5: Tube 4
  • Lane 6: Tube 5
  • Lane 7: Tube 6
  • Lane 8: Tube 7
  • Lane 9: Tube 8
  • Lane 10: Tube 9
  • Lane 11: Tube 10
  • Lane 12: Tube 11
  • Lane 13: Tube 12

Discussion

The bands we got from the gel shows that digestion in tubes 1, 2, 4, 10, 11 and 12 worked.

Conclusion

We use the digestion from the six tubes that worked for ligation.

rocF and Subtilin immunity

Gel extraction

PCR

Newcastle 25810gel psb1c3.jpg





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