Team:Newcastle/25 August 2010

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(Difference between revisions)
(Restriction digestion and gel extraction linearized pSB1C3)
(First transformation of Bacillius subtilis 168 with Prrnb-GFP containing yneA)
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=First transformation of ''Bacillius subtilis'' 168 with Prrnb-GFP containing ''yneA''=
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=Transformation of ''Bacillius subtilis'' 168 with Prrnb-GFP containing ''yneA''=
==Aim==
==Aim==
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To transform ''yneA'' into competent ''B. subtilis''.
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We performed gel electrophoresis on our 12 digested minipreps and found 8 were the correct product. We transformed ''B. subtilis'' 168 with one of the samples (1).
==Materials and Protocol==
==Materials and Protocol==

Revision as of 01:00, 28 October 2010

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Contents

Transformation of Bacillius subtilis 168 with Prrnb-GFP containing yneA

Aim

We performed gel electrophoresis on our 12 digested minipreps and found 8 were the correct product. We transformed B. subtilis 168 with one of the samples (1).

Materials and Protocol

Please refer to transformation of B. subtilis.

Results and Conclusion

Please refer to results in tomorrow's lab book.