Team:Newcastle/24 August 2010
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=Miniprep for pGFPrrnB with ''yneA''= | =Miniprep for pGFPrrnB with ''yneA''= | ||
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+ | =Digestion of pGFPrrnB with insert ''yneA'' with hinDIII= | ||
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Revision as of 13:57, 24 August 2010
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Contents |
Miniprep for pGFPrrnB with yneA
Aims
To produce more stocks of vector pGFPrrnB with insert yneA.
Materials and Protocol
Please refer to qiagen minipreps and nanodrop spectrophotometer for all 12 tubes of miniprep that we did.
Results
The results from the nanodrop:
Discussion
The results from the nanodrop showed that we have produced high concentration of vector pGFPrrnB with insert yneA. We will then proceed to digestion.