Team:Newcastle/23 June 2010

From 2010.igem.org

(Difference between revisions)
(Wednesday afternoon taking a break)
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===Wednesday Morning===
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Cells are made competent ready for transformation.
 +
*Positive control(plasmid uncut)- to make sure cells are transfroming properly
 +
*Negative control (just cells)- nothing but competent cells
 +
 +
5 microfuge tubes:
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*1:5
 +
*1:3
 +
*Vector
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*Negative add nothing
 +
*Positive add 1μl uncut
 +
=== Wednesday afternoon taking a break ===
=== Wednesday afternoon taking a break ===
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|[[Image:Newcastle_Hoppings_001.JPG|thumb]]
|[[Image:Newcastle_Hoppings_001.JPG|thumb]]
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|[[Image:Newcastle_Hoppings_002.JPG|thumb]]
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|[[Image:Newcastle_Hoppings_002.JPG|thumb|Call to arms!]]
|[[Image:Newcastle_Hoppings_003.JPG|thumb|Philamentous]]
|[[Image:Newcastle_Hoppings_003.JPG|thumb|Philamentous]]
|[[Image:Newcastle_Hoppings_004.JPG|thumb]]
|[[Image:Newcastle_Hoppings_004.JPG|thumb]]

Revision as of 13:46, 24 June 2010

Wednesday Morning

Cells are made competent ready for transformation.

  • Positive control(plasmid uncut)- to make sure cells are transfroming properly
  • Negative control (just cells)- nothing but competent cells

5 microfuge tubes:

  • 1:5
  • 1:3
  • Vector
  • Negative add nothing
  • Positive add 1μl uncut

Wednesday afternoon taking a break

Newcastle Hoppings 001.JPG
Call to arms!
Philamentous
Newcastle Hoppings 004.JPG
Newcastle Hoppings 005.JPG
Newcastle Hoppings 006.JPG
Newcastle Hoppings 008.JPG
Newcastle Hoppings 009.JPG
Newcastle Hoppings 010.JPG
Newcastle Hoppings 011.JPG
Newcastle Hoppings 012.JPG
Newcastle Hoppings 013.JPG
Newcastle Hoppings 014.JPG
Newcastle Hoppings 015.JPG
Newcastle Hoppings 016.JPG
Newcastle Hoppings 017.JPG
Newcastle Hoppings 018.JPG
Newcastle Hoppings 019.JPG
Newcastle Hoppings 022.JPG
Newcastle Hoppings 024.JPG