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From 2010.igem.org

Monday

Wet Lab techniques practice

Tips:

• Treat everything as a pathogen!
• Work around the bunsen burner.
• Use a control.
• Clean the bench at the end of the day!
• Heat the wire loop from the middle to the tip.
• Keep plates upside down.
• Keep lids off for as short a time as possible.
• Loosen all the tops off the vessels before you start.
• Flame tops of bottles lightly.

Streak plating

Star plate streaking student, Deena, shows off how it is to be done!

Set up culture for minipreps

5ml culture with E.coli from a single colony grown over night. Tomorrow we will harvest the DNA.