"
Team:Newcastle/21 June 2010
From 2010.igem.org
(Difference between revisions)
Shethharsh08 (Talk | contribs) |
|||
| Line 1: | Line 1: | ||
{{Team:Newcastle/mainbanner}} | {{Team:Newcastle/mainbanner}} | ||
| - | ''' | + | This is the '''first day''' of the iGEM lab training. We were given several important tips by Dr. Wendy Smith to begin with, and then throughout the day, we were familiarized with basic lab techniques, e.g. the use of pipettes. |
| - | |||
==Wet Lab techniques practice== | ==Wet Lab techniques practice== | ||
===Aims=== | ===Aims=== | ||
| - | The aim of today's Lab training session was to practice aseptic | + | The aim of today's Lab training session was to practice aseptic techniques and wet lab techniques such as streak plating and broth culture preparation. |
| - | === | + | ===Materials Required=== |
'''For today's session we needed''': | '''For today's session we needed''': | ||
| Line 20: | Line 19: | ||
* Bunsen burner | * Bunsen burner | ||
* Conical flasks | * Conical flasks | ||
| - | * Orbital shaker | + | * Orbital shaker |
| + | |||
===List of techniques=== | ===List of techniques=== | ||
| - | # | + | #Used electronic balance and made LB broth. |
| - | # | + | #Prepared broth culture |
| + | #Familiarized with using pipettes of different volumes | ||
#Mini-Prep introduction for Tuesday. | #Mini-Prep introduction for Tuesday. | ||
| - | |||
| - | The biobrick '''BBa_J04450''''s prefix and suffix were identified. They are EcoRI and PstI respectively. | + | The biobrick '''BBa_J04450''''s prefix and suffix were identified from the parts registry. They are EcoRI and PstI respectively. |
===Tips=== | ===Tips=== | ||
* Use aseptic technique: Treat everything as a pathogen! | * Use aseptic technique: Treat everything as a pathogen! | ||
| - | * Work around | + | * Work around burnsen burner. |
| - | * Use a control. | + | * Use a control for every experiment. |
| - | * Clean the bench at the end of the day | + | * Clean the bench at the end of the day. |
| - | * Heat the | + | * Heat the flame loop from the middle to the tip till it becomes red hot. |
| - | * Keep plates upside down. | + | * Keep plates upside down so that condensation does not damage the colonies. |
| - | * Keep lids off for as short a time as possible. | + | * Keep the lids of the plates off for as short a time as possible. |
* Loosen all the tops off the vessels before you start. | * Loosen all the tops off the vessels before you start. | ||
| - | * Flame tops of bottles lightly. | + | * Flame tops of bottles lightly so as to reduce the chances of contamination. |
| - | * Colony plates should be | + | * Colony plates should be labeled (name of culture, our initials and date) at the base in order to prevent condensation. |
| - | * For individual | + | * For individual colony selection, steak across a few times in different directions in order to dilute the number of colonies per unit area. |
| - | * Wear gloves, especially when working with DNA, PCR etc. However, never wear gloves when working near a flame | + | * Wear gloves, especially when working with DNA, PCR etc. However, never wear gloves when working near a flame. |
| + | |||
===Streak plating=== | ===Streak plating=== | ||
Revision as of 13:25, 2 August 2010
| |||||||||||||
| |||||||||||||
This is the first day of the iGEM lab training. We were given several important tips by Dr. Wendy Smith to begin with, and then throughout the day, we were familiarized with basic lab techniques, e.g. the use of pipettes.
Contents |
Wet Lab techniques practice
Aims
The aim of today's Lab training session was to practice aseptic techniques and wet lab techniques such as streak plating and broth culture preparation.
Materials Required
For today's session we needed:
- Agar plates
- Pipettes
- Wire loops
- E.coli (from a colony)
- LB broth
- Bunsen burner
- Conical flasks
- Orbital shaker
List of techniques
- Used electronic balance and made LB broth.
- Prepared broth culture
- Familiarized with using pipettes of different volumes
- Mini-Prep introduction for Tuesday.
The biobrick BBa_J04450's prefix and suffix were identified from the parts registry. They are EcoRI and PstI respectively.
Tips
- Use aseptic technique: Treat everything as a pathogen!
- Work around burnsen burner.
- Use a control for every experiment.
- Clean the bench at the end of the day.
- Heat the flame loop from the middle to the tip till it becomes red hot.
- Keep plates upside down so that condensation does not damage the colonies.
- Keep the lids of the plates off for as short a time as possible.
- Loosen all the tops off the vessels before you start.
- Flame tops of bottles lightly so as to reduce the chances of contamination.
- Colony plates should be labeled (name of culture, our initials and date) at the base in order to prevent condensation.
- For individual colony selection, steak across a few times in different directions in order to dilute the number of colonies per unit area.
- Wear gloves, especially when working with DNA, PCR etc. However, never wear gloves when working near a flame.
Streak plating
Set up culture for minipreps
5 ml culture with E.coli from a single colony grown over night. Tomorrow we will harvest the DNA.
Outcome
We learnt aseptic technique and some basic wetlab techniques. As you can see in the picture above our streak plates worked.
   
|
    
|
