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Team:Newcastle/21 June 2010
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RachelBoyd (Talk | contribs) (→Wet Lab techniques practice) |
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===Wet Lab techniques practice=== | ===Wet Lab techniques practice=== | ||
'''Tips''': | '''Tips''': | ||
| - | * Treat everything as a pathogen! | + | * Use Aseptic technique: Treat everything as a pathogen! |
* Work around the bunsen burner. | * Work around the bunsen burner. | ||
* Use a control. | * Use a control. | ||
| Line 12: | Line 12: | ||
* Keep lids off for as short a time as possible. | * Keep lids off for as short a time as possible. | ||
* Loosen all the tops off the vessels before you start. | * Loosen all the tops off the vessels before you start. | ||
| - | * Flame tops of bottles lightly. | + | * Flame tops of bottles lightly. |
| + | |||
===Streak plating=== | ===Streak plating=== | ||
[[Image:Newcastle_Deena_streakplate.JPG|alt="|Star plate streaking student, Deena, shows off how it is to be done!"|Caption="Star plate streaking student, Deena, shows off how it is to be done!"]] | [[Image:Newcastle_Deena_streakplate.JPG|alt="|Star plate streaking student, Deena, shows off how it is to be done!"|Caption="Star plate streaking student, Deena, shows off how it is to be done!"]] | ||
Revision as of 13:50, 24 June 2010
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Monday
Wet Lab techniques practice
Tips:
- Use Aseptic technique: Treat everything as a pathogen!
- Work around the bunsen burner.
- Use a control.
- Clean the bench at the end of the day!
- Heat the wire loop from the middle to the tip.
- Keep plates upside down.
- Keep lids off for as short a time as possible.
- Loosen all the tops off the vessels before you start.
- Flame tops of bottles lightly.
Streak plating
Star plate streaking student, Deena, shows off how it is to be done!
Set up culture for minipreps
5ml culture with E.coli from a single colony grown over night. Tomorrow we will harvest the DNA.
   
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