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Team:Newcastle/21 June 2010
From 2010.igem.org
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===Wet Lab techniques practice=== | ===Wet Lab techniques practice=== | ||
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5ml culture with ''E.coli'' from a single colony grown over night. | 5ml culture with ''E.coli'' from a single colony grown over night. | ||
Tomorrow we will harvest the DNA. | Tomorrow we will harvest the DNA. | ||
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Revision as of 13:49, 24 June 2010
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Monday
Wet Lab techniques practice
Tips:
- Treat everything as a pathogen!
- Work around the bunsen burner.
- Use a control.
- Clean the bench at the end of the day!
- Heat the wire loop from the middle to the tip.
- Keep plates upside down.
- Keep lids off for as short a time as possible.
- Loosen all the tops off the vessels before you start.
- Flame tops of bottles lightly.
Streak plating
Star plate streaking student, Deena, shows off how it is to be done!
Set up culture for minipreps
5ml culture with E.coli from a single colony grown over night. Tomorrow we will harvest the DNA.
   
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