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Contents 1 Aims 1.1 Materials and protocol 2 Gel Extraction 2.1 Aims 2.2 Materials and Protocol

Aims

Following yesterday's Phusion PCR, we plan to first perform gel electrophoresis, then gel extraction, and finally nanodrop to record the level of DNA.

Materials and protocol

Please refer to the gel electrophoresis, gel extraction and NanoDrop protocols. Vacuum manifold will be used.

Gel Extraction

Aims

To purify the PCR products of digested yneA, pGFPrrnB and pSB1C3 from yesterday.

Materials and Protocol

Please refer to gel extraction.