"
Team:Newcastle/20 August 2010
From 2010.igem.org
(Difference between revisions)
(→Aims) |
|||
| Line 59: | Line 59: | ||
==Aims== | ==Aims== | ||
| - | To ligate ''yneA'' with pGFPrrnB and ''yneA'' with pSB1C3. | + | To ligate ''yneA'' with pGFPrrnB and ''yneA'' with pSB1C3 (A repeat of [[Team:Newcastle/19_August_2010|yesterday]]. |
==Materials and Protocol== | ==Materials and Protocol== | ||
Revision as of 11:17, 20 August 2010
| |||||||||||||
| |||||||||||||
Contents |
Aims
Following yesterday's Phusion PCR, we plan to first perform gel electrophoresis, then gel extraction, and finally nanodrop to record the level of DNA.
Materials and protocol
Please refer to the gel electrophoresis, gel extraction and NanoDrop protocols. Vacuum manifold will be used.
Gel Extraction
Aims
To purify the PCR products of digested yneA, pGFPrrnB and pSB1C3 from yesterday.
Materials and Protocol
Please refer to gel extraction and nanodrop.
Results
Discussion
Conclusion
Miniprep of yneA, pGFPrrnB and pSB1C3
Aim
To produce stocks of yneA, pGFPrrnB and pSB1C3.
Materials and Protocol
Please refer to miniprep and nanodrop.
Results
Discussion
Conclusion
Transformation of Ligated Products
Aims
To produce more colonies of the ligated products of yneA with pGFPrrnB and pSB1C3.
Materials and Protocol
Please refer to transformation of E. coli.
Ligation of yneA with Vectors
Aims
To ligate yneA with pGFPrrnB and yneA with pSB1C3 (A repeat of yesterday.
Materials and Protocol
Please refer to ligation.
Results, Discussion and Conclusion
Please refer to 23.08.10.
   
|
    
|
