Team:Newcastle/20 August 2010

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{{Team:Newcastle/mainbanner}}
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=Transformation of ligated ''yneA'' into pGFPrrnB and pSB1C3=
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=Miniprep for ''yneA'', pGFPrrnB and pSB1C3=
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==Aim==
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The aim of this experiment is to produce stocks of ''yneA'', pGFPrrnB and pSB1C3.
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==Materials and Protocol==
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Please refer to [[Team:Newcastle/Qiagen_Minipreps|miniprep]] and [[TeamNewcastleNanoDrop_Spectrophotometer|nanodrop]].
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==Results==
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{|border=1
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|-
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! Sample no.
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! ''yneA''
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! pGFPrrnB
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! pSB1C3
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!1
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!432.5
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!238.9
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!126.1
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!2
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!347.6
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!230.7
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!107.6
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!3
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!380.2
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!390.9
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!121.5
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!4
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!377.9
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!236.2
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!112.8
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|}
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'''Table 1''': Nanodrop spectrophotometer results. Table represents the amount of plasmid present in µl/ml quantity.
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===Discussion===
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Results from the NanoDrop show concentration values of more than 100 ng/µl, which means we have obtained good concentration of DNA from the miniprep.
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===Conclusion===
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We keep the miniprep products as stocks for future use.
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==Transformation of ligated ''yneA'' into pGFPrrnB and pSB1C3==
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==Aims==
==Aims==
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Please refer to [[Team:Newcastle/Transformation_of_E._coli|transformation of E. coli]].
Please refer to [[Team:Newcastle/Transformation_of_E._coli|transformation of E. coli]].
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==Ligation==
 
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===Aims===
 
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To ligate ''yneA'' with pGFPrrnB and ''yneA'' with pSB1C3 (A repeat of [[Team:Newcastle/19_August_2010|yesterday]]).
 
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===Materials and Protocol===
 
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Please refer to [[Team:Newcastle/Ligation|ligation]].
 
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===Results, Discussion and Conclusion===
 
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Please refer to [[Team:Newcastle/23_August_2010|23.08.10]].
 
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==Plating ''Bacillus subtilis'' BFS678==
 
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Has pMutin4 (and thus ''lacI'') integrated into the chromosome...will be useful for characterisation.. will be transforming this strain with our filamentous cell and arginase BioBricks.
 
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 00:33, 28 October 2010

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Transformation of ligated yneA into pGFPrrnB and pSB1C3

Aims

To produce more colonies of the plasmid pGFPrrnB and pSB1C3 containing yneA with pGFPrrnB and pSB1C3 fromyesterday.

Materials and Protocol

Please refer to transformation of E. coli.


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