Team:Newcastle/1 September 2010

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Bacillus sphaericus Rehydration

Aims

We aimed to rehydrate and plate the natto strain (Bacillus subtilis ATCC 31578) bought from DSMZ, Germany. We used Penassay G- Thy Medium to rehydrate the cells. The cells are going to be used to test the strength of PGA glue.

Materials

  • Pennassay G- Thy medium:
  1. Pennassay broth- 17.5g
  2. Glucose- 20g
  3. Thymine- 0.05g
  4. Distilled water- 1000ml
  • Pennassay broth( per l of water):
  1. peptone- 10g
  2. Yeast extract- 1.5g
  3. Sodium chloride- 3.5g
  4. Glucose- 1.0g
  5. Potassium phosphate dibasic- 3.68g
  6. Potassium phospahte monobasic- 1.32g
  7. Beef extract- 1.5g

ph7 store at 2-8 °C


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