Team:Newcastle/1 July 2010

From 2010.igem.org

(Difference between revisions)
 
Line 24: Line 24:
*To make a stock of competent ''E. coli'' (DH5α strain) ready for transformation.
*To make a stock of competent ''E. coli'' (DH5α strain) ready for transformation.
-
==Materials==
+
==Materials and Protocol==
-
*''E. coli'' DH5α strain
+
-
 
+
-
==Protocol==
+
* Set up [[Team:Newcastle/Growing a liquid culture| liquid culture]] consisting of ''E. coli'' DH5α.
* Set up [[Team:Newcastle/Growing a liquid culture| liquid culture]] consisting of ''E. coli'' DH5α.
{{Team:Newcastle/footer}}
{{Team:Newcastle/footer}}

Latest revision as of 14:11, 25 October 2010

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Contents

Urease Test

Aims

The aim of this experiment was to determine if Bacillus subtilis 168 is able to produce urease and degrade urea.

Procedures

Inference

Christensen's Urea Agar was formulated to detect and differentiate urolytic and urea degrading microorganisms. The addition of gelatine peptone, dextrose and reduced content of buffer supports a luxuriant growth at an early stage. Urea is the substrate and can be degraded by certain organisms, which results in ammonia building. The ammonia makes the media alkaline and therefore the indicator phenol red will change from orange color to pink-red color.

  • Negative control - No color change (Orange color)
  • Test 1 (Duplicate) - Pink-red color
  • Test 2 (Duplicate) - Pink-red color

For actual results, please see 02.07.10 lab notebook.

Competent E. coli Production

Aims

  • To make a stock of competent E. coli (DH5α strain) ready for transformation.

Materials and Protocol


Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon