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Team:Newcastle/19 August 2010
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==Aims== | ==Aims== | ||
| - | To extract the correct size bands for ''yneA'', pGFPrrnB and PSB1AT3 from the gel after running gel electrophoresis. | + | To extract the correct size bands for ''yneA'', pGFPrrnB and PSB1AT3 from the gel after running gel electrophoresis. Concentration of the DNA and vectors are also checked with nanodrop. |
==Materials and Protocol== | ==Materials and Protocol== | ||
| - | Please refer to [[Team:Newcastle/Gel_extraction|gel extraction]]. | + | Please refer to [[Team:Newcastle/Gel_electrophoresis|gel electrophoresis]], [[Team:Newcastle/Gel_extraction|gel extraction]], [[TeamNewcastleNanoDrop_Spectrophotometer|nanodrop spectrophotometer]]. |
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| + | ==Results== | ||
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| + | ==Discussion== | ||
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| + | The bands we extracted from the gel was good for pGFPrrnB, but not so strong for ''yneA'' and PSB1C3. | ||
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| + | The results we got from the nanodrop were not as good as expected for ''yneA'' and PSB1C3. | ||
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| + | ==Conclusion== | ||
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| + | We will proceed with digestion, but another set of ligation will be set up to repeat the process again. | ||
Revision as of 13:43, 19 August 2010
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Contents |
Gel extraction of yneA
Aims
To extract the correct size bands for yneA, pGFPrrnB and PSB1AT3 from the gel after running gel electrophoresis. Concentration of the DNA and vectors are also checked with nanodrop.
Materials and Protocol
Please refer to gel electrophoresis, gel extraction, nanodrop spectrophotometer.
Results
Discussion
The bands we extracted from the gel was good for pGFPrrnB, but not so strong for yneA and PSB1C3.
The results we got from the nanodrop were not as good as expected for yneA and PSB1C3.
Conclusion
We will proceed with digestion, but another set of ligation will be set up to repeat the process again.
Go back to our main Lab book page
   
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