Revision as of 01:41, 26 October 2010

Miniprep of yneA BioBrick

The aim of the experiment is to extract yneA in the biobrick comapatible vector from 17th August, 2010 overnight culture.

Please refer to protocols mentioned below for materials required:

	yneA 1	yneA 2	yneA 3	yneA 4
Concentration of DNA ng/µl	247.3	233.6	456.6	140.0

Table 1: Nanodrop spectrophotometer results. Table represents the amount of plasmid present in µl/ml quantity.

Go back to our main Lab book page

@@ Line 2: / Line 2: @@
-=''yneA''=
+=Miniprep of ''yneA'' BioBrick=
 ==Aims==
-Today we aim to extract ''yneA'' in the biobrick comapatible vector from yesterday's overnight culture. We aim to check the concentration from the miniprep with the nanodrop then digest the plasmid with EcoR1 and Nhe1, ligate into pGFPrrnB and transform cells to grow on a plate overnight.
+The aim of the experiment is to extract ''yneA'' in the biobrick comapatible vector from 17th August, 2010 overnight culture.
 ==Materials and Protocol==
@@ Line 13: / Line 12: @@
 *[[Team:Newcastle/Qiagen_Minipreps|Plasmid extraction protocol]]
 *[[TeamNewcastleNanoDrop_Spectrophotometer|Nanodrop protocol]]
-*[[Team:Newcastle/Restriction_digests|Restriction digest protocol]]
-*[[Team:Newcastle/Gel_electrophoresis|Gel electrophoresis protocol]]
-*[[Team:Newcastle/Gel_extraction| Gel extraction protocol]]
 ==Results==
-===Nanodrop results===
 {|border=1
 |-
@@ Line 35: / Line 29: @@
 |}
+'''Table 1''': Nanodrop spectrophotometer results. Table represents the amount of plasmid present in µl/ml quantity.
 [[Image:Nanodrop1882010.jpeg|350px]]