Team:Newcastle/16 July 2010

From 2010.igem.org

Revision as of 10:50, 5 August 2010 by PhilipHall (Talk | contribs)

iGEM Homepage Newcastle University BacillaFilla Homepage Image Map

Screening for lacI insert

Aim

The aim of this part of this experiment is to screen for the lacI insert.

Protocol

    1. Miniprep - the miniprep protocol was followed.
    2. Restriction digests - the restriction digests protocol was followed to allow the length of the DNA to be checked using single and double digest.
      • Single digest with Pst1
      • double digests with Pst1 and Xba1
    3. Gel electrophoresis - the gel electrophoresis protocol was followed.

Inference

  • Set up liquid broth culture in LB
Newcastle University logo.png    Newcastle cbcb logo.pngNewcastle Biomedicine logo.gif    Team Newcastle CEG logo.gif
Newcastle iww logo.jpg  UNIPV Pavia Logo.gif  Newcastle BBSRC.gif    Newcastle Genevision logo.png Newcastle WelcomeTrust.jpg
FaceBook Icon